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LIVE IMAGING OF i3NEURONS (Support Protocol 5) v1

Year: 2019

Type: preprint

Abstract: Live imaging permits visualization of molecular and organellar dynamics within the neuron. While a standard confocal microscope is sufficient for short imaging experiments, extended imaging applications (>1 hr) are best served by a 37 °C live imaging chamber outfitted onto the microscope. CM should also be changed to Hibernate A Low Fluorescence Medium (BrainBits LLC, cat. no. SKU#HAPR) for extended imaging.

DOI: 10.17504/protocols.io.5w7g7hn

Authors: Michael S. Fernandopulle, Ryan Prestil, Christopher Grunseich, Chao Wang, Li Gan, Michael E. Ward

Institutions: National Institutes of Health, Gladstone Institutes, National Institute of Neurological Disorders and Stroke

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

OpenAlex ID: W3012473906

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