Platelet-rich plasma promotes cell viability of human hair dermal papilla cells (HHDPCs) in vitro.
Year: 2017
Type: article
Abstract: PURPOSE: This study aimed to clarify the effect of platelet-rich plasma (PRP) on human hair dermal papilla cell (HHDPC) viability and expression levels of alkaline phosphatase (ALP) and versican. METHODS: Eight healthy volunteers were enrolled, and different concentrations of PRP and platelet-poor plasma (PPP) were preparedby 2-step centrifugation method. HHDPCs were thencultivated. To identify the best incubating time of HHDPCs and plasmaconcentration, cell viability was investigated by MTT assays. In PRP- or PPP-treated HHDPCs, the mRNA levels of versican and ALP were checked by real-time quantitative PCR (qRT-PCR). RESULTS: Both PRP (5% and 10%) and PPP (5% and 10%) could significantly promote cell viability of the HHDPCs (p4) in 72 h group, while the effect of 10% PRP on HHDPC viability was less than that of 5% PRP. qRT-PCR showed that PRP could significantly increase mRNA levels of ALP and versican in HHDPCs (p4) when compared with the control group. Meanwhile,Western blot indicated an obviously increased protein level of ALP and versican in PRP-treated HHDPCs (p4). CONCLUSION: PRP could promote cell viability of HHDPCs. Besides, 5% PRP could significantly increase ALP and versican expression in both mRNA and protein levels in HHDPCs (p4).
Source: PubMed Central
Authors: Yuyan Wang, Liang Tang, Fei Zhu, Ming Jia
Language: English
Topic: Hair Growth and Disorders
Subfield: Dermatology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W3013254030