Direct Regeneration of Three Indian Maize Genotypes by Multiple Shoot Induction Using Split Nodes
Year: 2020
Type: article
Abstract: Regeneration protocol is pre-requisite for the development of transgenic plants. Maize (Zea mays L) crop is severely affected by fungal disease turcicum leaf blight. In the present study, standardizing the regeneration protocol for maize genotype was carried for the development of transgenic maize against turcicum leaf blight. The direct regeneration of maize involved nodal explants from the maize coleoptiles. Three Turcicum leaf blight susceptible maize genotypes (NAI179, NAI193, and HKI413) were selected for this study. Different combinations of growth regulators were used with MS media to induce shoots and roots. The best responding explants for the shooting was recorded in NAI179 on MS + 1.5mgL-1 BAP media. Fastest responding genotype was found to be HKI413 on MS + 0.5 mgL-1 BAP media. The maximum number of shoots were recorded in NAI179 on MS + mgL-1 BAP media and maximum shoot elongation was seen in NAI193 on MS + 2.5 mgL-1 BAP + 0.5 mgL-1 IBA. The best response for rooting was observed in NAI179 on MS + 0.5 mgL-1 BAP + 2.0 mgL-1 NAA media and fastest responding genotypes was found to be HKI413.
Authors: D. P. Adarsha, N. Nagesha
Institution: University of Agricultural Sciences, Bangalore
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W3013953227