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Development of a workflow for identification of nuclear genotyping markers for Cyclospora cayetanensis

Year: 2020
Type: article
Abstract: Cyclospora cayetanensis is an intestinal parasite responsible for the diarrheal illness, cyclosporiasis. Molecular genotyping, using targeted amplicon sequencing, provides a complementary tool for outbreak investigations, especially when epidemiological data are insufficient for linking cases and identifying clusters. The goal of this study was to identify candidate genotyping markers using a novel workflow for detection of segregating single nucleotide polymorphisms (SNPs) in C. cayetanensis genomes. Four whole C. cayetanensis genomes were compared using this workflow and four candidate markers were selected for evaluation of their genotyping utility by PCR and Sanger sequencing. These four markers covered 13 SNPs and resolved parasites from 57 stool specimens, differentiating C. cayetanensis into 19 new unique genotypes.
Source: Parasite
DOI: 10.1051/parasite/2020022
Authors: Katelyn A. Houghton, Alexandre Lomsadze, Subin Park, Fernanda S. Nascimento, Joel Barratt, Michael J. Arrowood, Erik VanRoey, Eldin Talundzic, Mark Borodovsky, Yvonne Qvarnstrom
Institutions: Centers for Disease Control and Prevention, Center for Global Health, Division of Parasitic Diseases and Malaria, Georgia Institute of Technology, The Wallace H. Coulter Department of Biomedical Engineering, National Center for Emerging and Zoonotic Infectious Diseases
Language: English
Subfield: Parasitology
Open Access status: gold
Estimated APC paid: $1,713
OpenAlex ID: W3015665239
FWCI: 2.435
Cites: 27
Cited by: 16
Related to: 10