Oleic acid Induces Tissue Resident FoxP3 Regulatory T cell Lineage Stability and Suppressive Functions
Year: 2020
Type: preprint
Abstract: ABSTRACT FoxP3 positive regulatory T cells (T regs ) rely on fatty acid β-oxidation (FAO)-driven OXPHOS for differentiation and function. Recent data have demonstrated a role for T regs in the maintenance of tissue homeostasis with tissue-resident T regs possessing tissue-specific transcriptomes. However, specific signals that establish these tissue-resident T regs programs are largely unknown. As T regs metabolically rely on FAO, and considering the lipid-rich environments of tissues, we hypothesized that environmental lipids drive T reg homeostasis. Using human adipose tissue as a model for tissue residency, we identify oleic acid as the most prevalent free fatty acid in human adipose tissue. Mechanistically, oleic acid amplifies T reg FAO-driven OXPHOS metabolism, creating a positive feedback mechanism that induces the expression of Foxp3 and enhances phosphorylation of STAT5, which acts to stabilize the T reg lineage and increase suppressive function. Comparing the transcriptomic program induced by oleic acid to that of the pro-inflammatory arachidonic acid, we find that T regs sorted from peripheral blood and adipose of healthy donors transcriptomically resemble the oleic acid in vitro treated T regs , whereas T regs obtained from the adipose tissue of relapsing-remitting MS patients more closely resemble an arachidonic acid profile. Finally, we find that oleic acid concentrations are reduced in the fat tissue of MS patients, and exposure of dysfunctional MS T regs to oleic acid restores defects in their suppressive function. These data demonstrate the importance of fatty acids in regulating tissue inflammatory signals.
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
Funder: Yale University
OpenAlex ID: W3016441684