Real-time, functional intra-operative localization of rat cavernous nerve network using near-infrared cyanine voltage-sensitive dye imaging
Year: 2020
Type: article
Abstract: Despite current progress achieved in the surgical technique of radical prostatectomy, post-operative complications such as erectile dysfunction and urinary incontinence persist at high incidence rates. In this paper, we present a methodology for functional intra-operative localization of the cavernous nerve (CN) network for nerve-sparing radical prostatectomy using near-infrared cyanine voltage-sensitive dye (VSD) imaging, which visualizes membrane potential variations in the CN and its branches (CNB) in real time. As a proof-of-concept experiment, we demonstrate a functioning complex nerve network in response to electrical stimulation of the CN, which was clearly differentiated from surrounding tissues in an in vivo rat prostate model. Stimulation of an erection was confirmed by correlative intracavernosal pressure (ICP) monitoring. Within 10 minutes, we performed trans-fascial staining of the CN by direct VSD administration. Our findings suggest the applicability of VSD imaging for real-time, functional imaging guidance during nerve-sparing radical prostatectomy.
Source: Scientific Reports
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,870
Funders: U.S. Department of Defense, U.S. Department of Health and Human Services, National Research Foundation, National Research Foundation of Korea, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Mental Health, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Biomedical Imaging and Bioengineering, Congressionally Directed Medical Research Programs
Awards: Early-Stage Prostate Cancer Screening Using Contrast-Enhanced Molecular Photoacoustic Imaging, EARLY-STAGE PROSTATE CANCER SCREENING USING CONTRAST-ENHANCED MOLECULAR PHOTOACOUSTIC IMAGING, G3447280225, G4119534169, G4703771726, G5129605863, G5342976984, G5850682332, Dysregulatory NOS Nexus for Erectile and Voiding Disorders, G6721446040, G7247525055, G8166743225, Potentiometric dyes and membrane permeability, G9000085048, Imaging in vivo neurotransmitter modulation of brain network activity in realtime
OpenAlex ID: W3016553453