Mp16-19 Modeling the Impact of Inflammatory Bowel Disease on Prostate Cancer Risk in Mice: Preliminary Results of an Ongoing Study
Year: 2020
Type: article
Study design: Observational Study
Abstract: You have accessJournal of UrologyProstate Cancer: Basic Research & Pathophysiology I (MP16)1 Apr 2020MP16-19 MODELING THE IMPACT OF INFLAMMATORY BOWEL DISEASE ON PROSTATE CANCER RISK IN MICE: PRELIMINARY RESULTS OF AN ONGOING STUDY Anuj Desai*, Barbara Lysy, Vinay Sagar, Rajita J Vatapalli, HuiYing Han, Kenji Unno, Yara R Rodriguez, Conor Driscoll, Jenny Ross, Jennifer D Wu, Sarki A Abdulkadir, and Shilajit Kundu Anuj Desai*Anuj Desai* More articles by this author , Barbara LysyBarbara Lysy More articles by this author , Vinay SagarVinay Sagar More articles by this author , Rajita J VatapalliRajita J Vatapalli More articles by this author , HuiYing HanHuiYing Han More articles by this author , Kenji UnnoKenji Unno More articles by this author , Yara R RodriguezYara R Rodriguez More articles by this author , Conor DriscollConor Driscoll More articles by this author , Jenny RossJenny Ross More articles by this author , Jennifer D WuJennifer D Wu More articles by this author , Sarki A AbdulkadirSarki A Abdulkadir More articles by this author , and Shilajit KunduShilajit Kundu More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000841.019AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Recent epidemiological data has implicated inflammatory bowel disease (IBD) as a risk factor for clinically significant prostate cancer (PC). One key driver of malignant transformation may include longstanding chronic inflammation, however, the local effects of gut inflammation on the prostate are unknown. Using a well-established murine model for chronic colitis, we are assessing whether gut inflammation leads to changes in the inflammatory milieu in the prostate of wild-type mice and transgenic prostate cancer-prone Pten-knockout mice. Here, we report preliminary results of a pilot study that is currently being validated in a larger cohort. METHODS: Chronic colitis was induced by administering C57BL/6 mice with 3 cycles of 3% dextran-sodium sulfate (DSS) – treated water for 7 days followed by 14 days of rest. Preliminary histologic analysis has been performed on DSS-treated mice gut and prostate tissue with independent pathologist review. Relative mRNA expression of prostatic inflammatory cytokines was obtained by quantitative real-time PCR. A similar experiment is currently ongoing with cohorts of both wild-type and transgenic Pten knockout mice (n=5-7/group). RESULTS: DSS-treated mice exhibited bloody diarrhea and weight loss with progression of colitis. Histologic analysis of the colon demonstrated marked epithelial erosion and infiltration of granulocytes into submucosa. In the prostate, DSS-treated mice had relatively higher expression of CD45 and cleaved caspase-3 compared to the control. In addition, TNF-alpha mRNA in the prostate was 6-fold higher in treated mice compared to the control. CONCLUSIONS: Preliminary data from this ongoing study suggests that induction of chronic colitis in mice is associated greater immune-cell infiltrate and higher inflammatory cytokine profile in the prostate. We anticipate that ongoing work from this study will better help to elucidate potential mechanisms of prostate tumorigenesis in men with IBD. Source of Funding: SPORE in Prostate Cancer © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e222-e223 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Anuj Desai* More articles by this author Barbara Lysy More articles by this author Vinay Sagar More articles by this author Rajita J Vatapalli More articles by this author HuiYing Han More articles by this author Kenji Unno More articles by this author Yara R Rodriguez More articles by this author Conor Driscoll More articles by this author Jenny Ross More articles by this author Jennifer D Wu More articles by this author Sarki A Abdulkadir More articles by this author Shilajit Kundu More articles by this author Expand All Advertisement PDF downloadLoading ...
Source: The Journal of Urology
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funder: Astellas Pharma
OpenAlex ID: W3021851438