Work

Generation of a human iPSC line (MPIi006-A) from a patient with Pelizaeus-Merzbacher disease

Year: 2020

Type: article

Abstract: We established a human induced pluripotent stem cells (hiPSC) line (MPIi006-A) from fibroblasts of a 20-year-old male Pelizaeus-Merzbacher disease (PMD) patient with a hemizygous 643C>T mutation in proteolipid protein 1 (PLP1) gene using a retroviral delivery of OCT4, SOX2, KLF4 and c-MYC. The MPIi006-A iPSC line carried the mutation, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited normal karyotype and were capable of differentiating into cells representative of three germ layers.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2020.101839

Authors: Kee-Pyo Kim, Juyong Yoon, Borami Shin, Albrecht Röpke, Dong Wook Han, Hans R. Schöler

Institutions: Max Planck Institute for Molecular Biomedicine, University of Münster, Wuyi University, Wuyi University

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,100