Pd27-05 Polarization of Macrophages From M0 to M2 When Seeded Upon Genipin-crosslinked Collagen Mesh for Treatment of Stress Urinary Incontinence
Year: 2020
Type: article
Study design: Clinical Trial
Abstract: You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Female Incontinence: Therapy II (PD27)1 Apr 2020PD27-05 POLARIZATION OF MACROPHAGES FROM M0 TO M2 WHEN SEEDED UPON GENIPIN-CROSSLINKED COLLAGEN MESH FOR TREATMENT OF STRESS URINARY INCONTINENCE Ilaha Isali*, Phillip McClellan, Eswar Shankar, Sanjay Gupta, Mukesh Jain, Ozan Akkus, and Adonis Hijaz Ilaha Isali*Ilaha Isali* More articles by this author , Phillip McClellanPhillip McClellan More articles by this author , Eswar ShankarEswar Shankar More articles by this author , Sanjay GuptaSanjay Gupta More articles by this author , Mukesh JainMukesh Jain More articles by this author , Ozan AkkusOzan Akkus More articles by this author , and Adonis HijazAdonis Hijaz More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000884.05AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The immunological response of the body to implanted biomaterials remains a significant issue. Several important urogynecological applications (Stress Urinary Incontinence, Pelvic Organ Prolapse) require tissue support meshes. These scenarios call for materials that promote de novo collagen deposition by the body. A key regulator of collagen production is pro-regenerative M2 macrophages. The creation of advanced biomaterials that encourage monocytes to polarize to M2 macrophages may be an effective way to increase surgical outcomes in tissue reinforcing mesh applications. Our past in vivo study has shown that M2 was greater with genipin implanted group [1]. Based on those results, we hypothesized that a genipin-crosslinked collagen mesh will promote polarization to M2. Our aims are: 1) to demonstrate the feasibility of seeding macrophages on genipin-crosslinked mesh for implantation; 2) to illustrate M0 macrophages on said scaffolds undergo a transition to M2 macrophages. METHODS: Monocytes were harvested from rat bone-marrow and macrophage subtypes were identified by using Flow Cytometry and immunocytochemistry using antibodies against CD68, CD163, CD86, Arginase 1, and iNOS. Electrochemically aligned collagen threads were filament wound to produce small collagen scaffolds, which were then crosslinking using genipin (Fig 1). Following sterilization, scaffolds were seeded with macrophages and cultured for up to 3 days. Experimental groups included M0 (N=3), M1 (N=3), and M2 (N=3) macrophages. Seeded scaffolds were assessed for cell attachment, proliferation, structure, and protein expression.Western blot for iNOS and Arginase 1 was performed on the macrophages from each group to determine their phenotype at 72 hours. RESULTS: Macrophages from all groups exhibited over 70% cell attachment at 4 hours following seeding and cell proliferation was similar among all groups. Protein expression highlighted the presence of arginase I and absence of iNOS in M0 cells at 72 hours after seeding on genipin-crosslinked fibers. CONCLUSIONS: Genipin-crosslinked collagen scaffolds support sufficient macrophage attachment and survival for implantation. Protein expression results suggest genipin-crosslinked collagen scaffolds induce polarization of M0 macrophages to an M2 phenotype.Reference:1.J Biomed Mater Res B Appl Biomater. 2019 Apr;107(3):479-489. doi: 10.1002/jbm.b.34138. Source of Funding: NIH grants:R21HD095439-01 and R01AR063701 © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e550-e551 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Ilaha Isali* More articles by this author Phillip McClellan More articles by this author Eswar Shankar More articles by this author Sanjay Gupta More articles by this author Mukesh Jain More articles by this author Ozan Akkus More articles by this author Adonis Hijaz More articles by this author Expand All Advertisement PDF downloadLoading ...
Source: The Journal of Urology
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W3022102688