Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer
Year: 2014
Type: article
Abstract: High-throughput production of nanoparticles (NPs) with controlled quality is critical for their clinical translation into effective nanomedicines for diagnostics and therapeutics. Here we report a simple and versatile coaxial turbulent jet mixer that can synthesize a variety of NPs at high throughput up to 3 kg/d, while maintaining the advantages of homogeneity, reproducibility, and tunability that are normally accessible only in specialized microscale mixing devices. The device fabrication does not require specialized machining and is easy to operate. As one example, we show reproducible, high-throughput formulation of siRNA-polyelectrolyte polyplex NPs that exhibit effective gene knockdown but exhibit significant dependence on batch size when formulated using conventional methods. The coaxial turbulent jet mixer can accelerate the development of nanomedicines by providing a robust and versatile platform for preparation of NPs at throughputs suitable for in vivo studies, clinical trials, and industrial-scale production.
Source: ACS Nano
DOI: 10.1021/nn501371n
Authors: Jong-Min Lim, Archana Swami, Laura M. Gilson, Sunandini Chopra, Sungyoung Choi, Jun Wu, Robert Langer, Rohit Karnik, Omid C. Farokhzad
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: Development of Disease-Specific Drug Delivery Vehicles, DF/HCC SPORE in Prostate Cancer, G4190385975, MIT-Harvard Center of Cancer Nanotechnology Excellence, Development of FcRn-Targeted Nanoparticles for Efficient Oral Delivery of Insulin, G6747909184, The MIT-Harvard Center of Cancer Nanotechnology Excelle*, G821760702, G9000153364, G9000231428, G947370982
OpenAlex ID: W2312592011