Mp10-19 Mature Randall’s Plaque Contains Cell Nuclei: Observations Using High-resolution Microscopy
Year: 2020
Type: article
Study design: Observational Study
Abstract: to understand how the microbiome may contribute to the onset of CaOx stones.METHODS: Two groups of patients, either with no history of USD or an active episode of CaOx stones, were recruited with five patients in each group.The metabolic potential of the urinary tract microbiome was quantified through comparative shotgun metagenomics from clean catch urine samples.Functional differences between groups were assessed through non-parametric t-tests of the normalized abundances of full-length genes, which were annotated through Prodigal and KEGG pathways.Additionally, draft genomes were extracted from the data to aid in future mechanistic studies.RESULTS: Shotgun metagenomics revealed that individuals with CaOx stones had a urinary tract microbiome distinct from those with no history of USD.CaOx stone formers were functionally less diverse than controls, corroborating previous metabolomic studies.Reduced functions of stone formers included oxalate metabolism, previously thought to be relegated to the gut microbiome, as well as transmembrane transport, proteolysis, and oxidation-reduction processes.A total of 17 draft genomes were extracted from the data, primarily from the Lactobacillus genus, which are thought protect against USD and urinary tract infections.CONCLUSIONS: Our prospective clinical study shows that individuals with USD harbor a distinct urinary tract microbiome.Surprisingly, USD patients harbored lower levels of oxalate-degrading genes, which suggests oxalate metabolism may continue in the urinary tract and play a role in inhibiting CaOx stone formation.Additional functions reduced in CaOx stone formers, such as proteolysis and oxidation-reduction processes overlap with known mechanisms of stone formation and thus may also play important roles for the onset of USD.
Source: The Journal of Urology
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W3023735464