Isolation of phytoconstituents and evaluation of anticancer and Antioxidant potential of Launaea mucronata (Forssk.) Muschl. subsp
Year: 2017
Type: article
Abstract: Traditional Medicine is widely used in clinical research due to its low toxicity, low number of side effects, and low cost. Many components of common fruits and vegetables play well-documented roles as chemo preventive or chemotherapeutic agents that suppress tumor genesis. The present study was aimed to identify the DPPH (2, 2-diphenyl1-picrylhydrazyl) free-radical scavenging constituents from methanol extract of Launaea mucronata using bioassayguided fractionation. The methanolic extract revealed a strong antioxidant activity, the IC50 values (the concentration with scavenging activity of 50%) was 25.94 mg/ml. The fractionation of the active methanol extract by silica gel column chromatography revealed compounds with strong antioxidant activity. The isolated bioactive compound was determined as, apigenin, quercetin, rutin, isorhamnetin-3-O-β-glucoside, quercetin-3-O-β-glucoside, apigenin-7-O- β-diglucoside, kaempferol-3-o-rutinoside), by comparing spectral data (UV, IR, 1 H NMR, 13C NMR, and MS) with literature reports. The results obtained confirmed that methanol extract of Launaea mucronata or its derived phytocompounds can be used potentially as a bioactive source of natural antioxidants by contributing beneficial health effects. Launaea mucronata was in vitro investigated for cytotoxic activity against HCT116, HepG2, Hella and MCF-7 cell lines, doxorubicin was used as the reference drug. The results show IC50 19, 19.60, 12.60 and 12.70 µg/ml respectively.
Author: Eman R Elsharkawy
Institutions: Northern Border University, Desert Research Center
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7153717655