Work

Kinetics and Optimization of the Lysine–Isopeptide Bond Forming Sortase Enzyme from Corynebacterium diphtheriae

Year: 2020

Type: article

Abstract: Site-specifically modified protein bioconjugates have important applications in biology, chemistry, and medicine. Functionalizing specific protein side chains with enzymes using mild reaction conditions is of significant interest, but remains challenging. Recently, the lysine–isopeptide bond forming activity of the sortase enzyme that builds surface pili in Corynebacterium diphtheriae ( Cd SrtA) has been reconstituted in vitro . A mutationally activated form of Cd SrtA was shown to be a promising bioconjugating enzyme that can attach Leu-Pro-Leu-Thr-Gly peptide fluorophores to a specific lysine residue within the N-terminal domain of the SpaA protein ( N SpaA), enabling the labeling of target proteins that are fused to N SpaA. Here we present a detailed analysis of the Cd SrtA catalyzed protein labeling reaction. We show that the first step in catalysis is rate limiting, which is the formation of the Cd SrtA-peptide thioacyl intermediate that subsequently reacts with a lysine ε-amine in N SpaA. This intermediate is surprisingly stable, limiting spurious proteolysis of the peptide substrate. We report the discovery of a new enzyme variant ( Cd SrtA Δ ) that has significantly improved transpeptidation activity, because it completely lacks an inhibitory polypeptide appendage (“lid”) that normally masks the active site. We show that the presence of the lid primarily impairs formation of the thioacyl intermediate and not the recognition of the N SpaA substrate. Quantitative measurements reveal that Cd SrtA Δ generates its cross-linked product with a catalytic turnover number of 1.4 ± 0.004 h –1 and that it has apparent K M values of 0.16 ± 0.04 and 1.6 ± 0.3 mM for its N SpaA and peptide substrates, respectively. Cd SrtA Δ is 7-fold more active than previously studied variants, labeling >90% of N SpaA with peptide within 6 h. The results of this study further improve the utility of Cd SrtA as a protein labeling tool and provide insight into the enzyme catalyzed reaction that underpins protein labeling and pilus biogenesis.

Source: Bioconjugate Chemistry

DOI: 10.1021/acs.bioconjchem.0c00163