Work

Unintended on-target chromosomal instability following CRISPR/Cas9 single gene targeting

Year: 2020

Type: article

Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) seminal studies in mammalian cells1–3 have resulted in gene editing being broadly adopted in basic research. However, it has become apparent that the CRISPR/Cas9 system induces unintended off- and on-target genomic alterations4 and that there is a need for stricter clone screening methods before phenotypic characterisation is made, particularly before the technology is adopted for clinical purposes.

Source: Annals of Oncology

DOI: 10.1016/j.annonc.2020.04.480

Authors: J. Przewrocka, A. Rowan, R. Rosenthal, N. Kanu, C. Swanton

Institutions: The Francis Crick Institute, University College London

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $2,528