Clusterin (apolipoprotein J) facilitates NF-κB and Bax degradation and prevents I/R injury in heart transplantation.
Year: 2018
Type: article
Abstract: BACKGROUND AND AIM: cell culture system and we examined the underlying mechanisms of I/R injury. METHODS: ischemia reperfusion model using H9C2 cells or H9C2/clusterin cDNA cells was constructed. The expression of clusterin, p65, Bax, Bcl-xL, IL-1β, and TNF-α protein and mRNA in heart tissue and H9C2 cells was detected by Western blot, reverse transcription-polymerase chain reaction (RT-PCR), and quantitative RT-PCR assays. IL-1β and TNF-α protein was detected by enzyme-linked immunosorbent assays. NF-kB activity was detected by an electrophoretic mobility shift assay and cell apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and flow cytometric analyses. RESULTS: . Clusterin inhibited cell apoptosis/death and reduced pre-inflammation. CONCLUSION: Clusterin is a promising target for preventing cold I/R injury in heart transplantation. This study also shows that the resultant protective effects of clusterin are mediated by NF-κB signaling and Bax/Bcl-xL expression.
Source: PubMed
Authors: Jiangdong Du, Liu Liu, Benxia Yu, Fengyun Hao, Guodong Liu, Hao Jing, Haiyan Xu
Institutions: Qingdao University, Yuhuangding Hospital
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W3027063079