Fluorescent Imaging for In Situ Measurement of Drug Target Engagement and Cell Signaling Pathways.
Year: 2020
Type: article
Abstract: hybridization of complementary oligonucleotides for biomarker labeling while oligonucleotide modifications facilitate signal removal for sequential rounds of fluorescent tagging and imaging. Ab-oligo CycIF is capable of generating extreme multi-parametric images for quantifying total and phosphorylated protein expression to quantify protein activation, expression, and spatial distribution. Together iPAI and Ab-oligo cycIF can be applied to interrogate drug uptake and target binding as well as changes to heterogenous cell populations within tumors that drive variable therapeutic responses in patients.
Source: PubMed
DOI: 10.1117/12.2546320
Authors: Nathan P McMahon, Allison Solanki, Jocelyn Jones, Sunjong Kwon, Young-Hwan Chang, Koei Chin, Michel A Nederlof, Joe W Gray, Summer L Gibbs
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
Funder: National Cancer Institute
OpenAlex ID: W3027837017