Year: 2019
Type: conference-paper
Abstract: Striatal cholinergic interneurons (CINs) were believed to function largely in a neuromodulatory fashion, acting on muscarinic receptors which have been demonstrated to regulate nearly all aspects of striatal functioning.Recently, it was demonstrated that they also exert fast nicotinic synaptic effects in striatal networks in particular by targeting several populations of GABAergic interneurons.This notion was first suggested by evidence demonstrating nicotinic activation of recurrent inhibition in CINs.Subsequently, we demonstrated that optogenetic activation of CINs elicits very large, disynaptic compound GABAergic IPSP/Cs in projection neurons that are secondary to nicotinic receptor activation.Using double transgenic preparations we showed that theses GABAergic responses elicited in projection neurons is mediated by nicotinic activation of striatal GABAergic interneurons.Since then, our recent data demonstrate that at least five different populations of striatal GABAergic interneurons receive nicotinic (as well as muscarinic) input from CINs.These include the tyrosine hydroxylase expressing interneurons (THINs), the NPYexpressing neurogliaform (NGF) and low-threshold spike interneurons (LTS), the fast adapting interneuron (FAI) and the spontaneously active bursty interneuron (SABI).Furthermore, in addition to innervating the striatal projection neurons, LTS, NGF and THINs also provide reciprocal connections to the CINs, which potentially put them in a central position in these circuits.Using double optogenetic strategy in multiple double transgenic mice we investigated the contribution of those GABAergic interneurons in the two nicotinic mediated striatal circuits described above i.e. the recurrent inhibitory circuit among striatal CINs and the nicotinic-mediated disynaptic inhibition of SPNs.Our results point to a specific and selective participation of several striatal GABAergic interneurons in these two nicotinic mediated striatal circuits.
Author: Rosario Moratalla
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3028412709