Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities
Year: 2020
Type: review
Abstract: , the endoplasmic reticulum and the mitochondria-forge highly-coordinated, particular environments essential for cross-compartment information flow. Our perspective summarizes chemical-biology tools that have enabled identifying proteins present within these itinerant communities against the bulk proteome, even when a particular protein's presence is fleeting/substoichiometric. However, proteins resident at these ephemeral junctions also experience transitory changes to their interactomes, small-molecule signalomes, and, importantly, functions. Thus, a thorough census of sub-organellar communities necessitates functionally probing context-dependent signaling properties of individual protein-players. Our perspective accordingly further discusses how repurposing of existing tools could allow us to glean a functional understanding of protein-specific signaling activities altered as a result of organelles pulling together. Collectively, our perspective strives to usher new chemical-biology techniques that could, in turn, open doors to modulate functions of specific subproteomes/organellar junctions underlying the nuanced regulatory subsystem broadly termed as contactology.
Source: RSC Chemical Biology
DOI: 10.1039/d0cb00041h
Authors: Marcus J. C. Long, Yi Zhao, Yimon Aye
Institution: École Polytechnique Fédérale de Lausanne
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,962
Funders: National Science Foundation, École Polytechnique Fédérale de Lausanne, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, National Center of Competence in Research Chemical Biology, National Institutes of Health, NIH Office of the Director, National Institute of General Medical Sciences
OpenAlex ID: W3029678772