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Maternal Choline Supplementation Supports Resistance to Effects of Prolonged Western Diet Feeding in Mice Offspring

Year: 2020

Type: article

Abstract: This study investigated whether maternal choline supplementation (MCS) could reduce the chronic metabolic stress that is induced by prenatal maternal obesity and postnatal Western Diet (WD) feeding. C57BL/6 dams were either fed a normal fat (NF, 10 kcal %fat) control diet or a high fat (HF, 45 kcal % fat) diet prior to, during pregnancy and during lactation. These dams received either 25 mM choline or plain drinking water. After weaning, offspring were fed the WD diet (45 kcal %fat, 34 kcal %fructose, and 0.25% cholesterol) for 16 weeks before glucose tolerance testing and dissection. After 16 weeks of WD feeding, offspring from normal-fat, choline supplemented fed (MCS-NF) dams are protected from weight gain compared to offspring from dams fed a high-fat diet with or without choline supplementation (MCS-HF or MCO-HF)(P < 0.043). Male offspring from MCS-NF fed dams have reduced caloric intake (P < 0.019) and reduced gene expression of Acc1 which mediates liver de novo fatty acid synthesis (P = 0.005) compared to the non-supplemented normal-fat dams (MCO-NF) after 16 weeks of WD feeding. Female offspring from MCS-HF fed dams have lower fasting glucose levels compared to MCO-HF fed dams after 16 weeks of WD feeding (P = 0.034). MCS mitigates some pathological hallmarks that are induced by prolonged WD feeding in offspring. NIGMS.

Source: Current Developments in Nutrition

DOI: 10.1093/cdn/nzaa054_094