Work

Uncovering the chemistry of C–C bond formation in C -nucleoside biosynthesis: crystal structure of a C -glycoside synthase/PRPP complex

Year: 2020

Type: article

Abstract: The enzyme ForT catalyzes C-C bond formation between 5'-phosphoribosyl-1'-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5'-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.

Source: Chemical Communications

DOI: 10.1039/d0cc02834g

Authors: Sisi Gao, Ashish Radadiya, Wenbo Li, Huanting Liu, Wen Zhu, Valérie de Crécy-Lagard, Nigel G. J. Richards, James H. Naismith

Institutions: University of St Andrews, Research Complex at Harwell, Cardiff University, University of Oxford, QB3, University of California, Berkeley, University of Florida, Foundation for Applied Molecular Evolution, Rosalind Franklin Institute

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Funders: Diamond Light Source, National Institutes of Health, Engineering and Physical Sciences Research Council, Biotechnology and Biological Sciences Research Council, National Institute of General Medical Sciences

Awards: Mapping C-C nucleoside bond formation in real time, Mapping C-C nucleoside bond formation in real time, Pyridoxal 5'-phosphate homeostasis in Escherichia coli, Structural Biology in the RFI

OpenAlex ID: W3033794541

FWCI: 1.936

Cites: 35

Cited by: 18

Related to: 10