Work

CRISPR Mediated Biosensing Toward Understanding Cellular Biology and Point‐of‐Care Diagnosis

Year: 2020

Type: article

Abstract: Recent advances in CRISPR based biotechnologies have greatly expanded our capabilities to repurpose CRISPR for the development of biomolecular sensors for diagnosing diseases and understanding cellular pathways. The key attribute that allows CRISPR to be widely utilized is the programmable and highly selective mechanism. In this Minireview, we first illustrate the molecular principle of CRISPR functioning process from sensing to actuating. Next, the CRISPR based biosensing strategies for nucleic acids, proteins and small molecules are summarized. We highlight some of recent advances in applications for in vitro detection of biomolecules and in vivo imaging of cellular networks. Finally, the challenges with, and exciting prospects of, CRISPR based biosensing developments are discussed.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.202005398

Authors: Yifan Dai, Yanfang Wu, Guozhen Liu, J. Justin Gooding

Institutions: Duke University, Case Western Reserve University, UNSW Sydney

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: University of New South Wales, Australian Research Council, Digital Grid Futures Institute, University of New South Wales Canberra

Awards: Intelligent nanoparticles: Interactive tools to decode brain activity, G441558795