Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology
Year: 2020
Type: article
Abstract: This work demonstrates a novel high-throughput (HT) microfluidics-enabled uninterrupted perfusion system (HT-μUPS) and validates its use with chronic all-optical electrophysiology in human excitable cells. HT-μUPS consists of a soft multichannel microfluidic plate cover which could button on a commercial HT 96-well plate. Herein, we demonstrate the manufacturing process of the system and its usages in acute and chronic all-optical electrophysiological studies of human induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CM) and engineered excitable (spiking HEK) cells. HT-μUPS perfusion maintained functional voltage and calcium responses in iPSC-CM and spiking HEK cells under spontaneous conditions and under optogenetic pacing. Long-term culture with HT-μUPS improved cell viability and optogenetically-tracked calcium responses in spiking HEK cells. The simplicity of this design and its compatibility with HT all-optical electrophysiology can empower cell-based assays for personalized medicine using patient-derived cells.
Source: Lab on a Chip
DOI: 10.1039/d0lc00615g
Authors: Lai Wei, Weizhen Li, Emilia Entcheva, Zhenyu Li
Institution: George Washington University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: hybrid
Awards: EFRI CEE: Human cardiac opto-epigenetics with HDAC inhibitors, On-chip optical biosensing methods for quantitative measurement of antibiotic resistance, G5070707301, PFI-TT: Automated Platform for Drug Testing in Human Heart Cells Using Light, Scalable platform for optimizing human cardiac tissue engineering via optical pacing and on-demand oxygenation
OpenAlex ID: W3087145856