An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laser
Year: 2020
Type: article
Abstract: An improved analysis for single-particle imaging (SPI) experiments, using the limited data, is presented here. Results are based on a study of bacteriophage PR772 performed at the Atomic, Molecular and Optical Science instrument at the Linac Coherent Light Source as part of the SPI initiative. Existing methods were modified to cope with the shortcomings of the experimental data: inaccessibility of information from half of the detector and a small fraction of single hits. The general SPI analysis workflow was upgraded with the expectation-maximization based classification of diffraction patterns and mode decomposition on the final virus-structure determination step. The presented processing pipeline allowed us to determine the 3D structure of bacteriophage PR772 without symmetry constraints with a spatial resolution of 6.9 nm. The obtained resolution was limited by the scattering intensity during the experiment and the relatively small number of single hits.
Source: IUCrJ
Authors: Dameli Assalauova, Young Yong Kim, Sergey Bobkov, Ruslan Khubbutdinov, Max Rose, Roberto Alvarez, Jakob Andreasson, Eugeniu Balaur, Alice Contreras, Hasan DeMirci, Luca Gelisio, Janos Hajdu, Mark S. Hunter, Ruslan P. Kurta, Haoyuan Li, Matthew McFadden, Reza Nazari, Peter Schwander, Anton Teslyuk, Peter Walter, P. Lourdu Xavier, Chun Hong Yoon, Sahba Zaare, Viacheslav A. Ilyin, Richard A. Kirian, Brenda G. Hogue, Andrew Aquila, Ivan A. Vartanyants
Institutions: Deutsches Elektronen-Synchrotron DESY, Kurchatov Institute, National Research Nuclear University MEPhI, Arizona State University, Czech Academy of Sciences, La Trobe University, ARC Centre of Excellence in Advanced Molecular Imaging, Koç University, SLAC National Accelerator Laboratory, Center for Free-Electron Laser Science, Uppsala University, European X-Ray Free-Electron Laser, Stanford University, University of Wisconsin–Milwaukee, Moscow Institute of Physics and Technology, Max Planck Institute for the Structure and Dynamics of Matter
Language: English
Subfield: Radiation
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,200
Funders: National Science Foundation, U.S. Department of Energy, Human Frontier Science Program, Joachim Herz Stiftung, Ministerstvo Školství, Mládeže a Tělovýchovy, Knut och Alice Wallenbergs Stiftelse, Vetenskapsrådet, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, Russian Science Foundation, Helmholtz Association, Röntgen-Ångström Cluster, Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Directorate for Biological Sciences, Office of Science, BioXFEL Science and Technology Center, European Research Council, European Regional Development Fund, Basic Energy Sciences, SLAC National Accelerator Laboratory
Awards: G1229526393, G1286251239, G1358207605, G176604203, G2371548206, G240744221, G2620015076, G262532246, G291036901, G303061748, G3490171961, G4235786860, G4280972364, G4473368111, G4652570887, G4654690619, G495301720, G498139845, G5570207115, G569772192, G5916014575, G6114423575, G6495240114, G6550344761, G6580471065, G6906370926, G7283024673, G7335658107, G7762871514, G7832026528, G7938408761, G8382000481, G8869491873, G911061179, Biology with X-ray Lasers, G969889393
OpenAlex ID: W3034212099