Work

Integrating CRISPR-Cas12a with a DNA circuit as a generic sensing platform for amplified detection of microRNA

Year: 2020

Type: article

Abstract: -cleavage activity of CRISPR-Cas12a for further signal amplification. Such rational integration provides a generic assay for the effectively amplified detection of miRNA biomarkers. By simply tuning the variable regions in the CHA modules, this assay achieves sub-femtomolar sensitivity for different miRNA biomarkers, which improves the detection limit of CRISPR-Dx in the analysis of miRNA by 3-4 orders of magnitude. With the usage of the proposed assay, the sensitive assessment of miR-21 levels in different cancer cell lines and clinical serum samples has been achieved, providing a generic method for the sensitive detection of miRNA biomarkers in molecular diagnosis.

Source: Chemical Science

DOI: 10.1039/d0sc03084h

Authors: Shuang Peng, Zhen Tan, Siyu Chen, Chunyang Lei, Zhou Nie

Institutions: Hunan University, State Key Laboratory of Chemobiosensing and Chemometrics

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: National Natural Science Foundation of China, Fundamental Research Funds for the Central Universities