Work

The laminin‐211‐derived PPFEGCIWN motif accelerates wound reepithelialization and increases phospho‐FAK‐Tyr397 and Rac1‐GTP levels in a rat excisional wound splinting model

Year: 2020

Type: article

Abstract: We previously reported that the PPFEGCIWN motif (Ln2-LG3-P2-DN3), residues 2678-2686 of the human laminin α2 chain, promotes cell attachment of normal human epidermal keratinocytes (NHEKs) and dermal fibroblasts (NHDFs); however, its in vivo effects on cutaneous wound healing have not yet been examined. In this study, we sought to determine whether Ln2-LG3-P2-DN3 could promote full-thickness cutaneous wound healing by accelerating wound reepithelialization and wound closure in vivo. Ln2-LG3-P2-DN3 had significantly higher cell attachment and spreading activities than vehicle or scrambled peptide control in both NHEKs and NHDFs in vitro. The wound area was significantly smaller in rats treated with Ln2-LG3-P2-DN3 than in those treated with vehicle or scrambled peptide in the early phase of wound healing. Furthermore, Ln2-LG3-P2-DN3 significantly accelerated wound reepithelialization relative to vehicle or scrambled peptide and promoted FAK-Tyr397 phosphorylation and Rac1 activation. Collectively, our findings suggest that the PPFEGCIWN motif has potential as a therapeutic agent for cutaneous regeneration via the acceleration of wound reepithelization and wound closure.

Source: Journal of Tissue Engineering and Regenerative Medicine

DOI: 10.1002/term.3084

Authors: Seung Bin Jo, Cho Yeon Park, Hyun Ki Kang, Sung Youn Jung, Byung‐Moo Min

Institutions: Seoul National University, Seoul National University Dental Hospital

Language: English

Topic: Wound Healing and Treatments

Subfield: Rehabilitation

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Research Foundation, National Research Foundation of Korea, Ministry of Science and ICT, South Korea