Work

Structure of the yeast Swi/Snf complex in a nucleosome free state

Year: 2020

Type: article

Abstract: SWI/SNF remodelers play a key role in regulating chromatin architecture and gene expression. Here, we report the cryo-EM structure of the Saccharomyces cerevisiae Swi/Snf complex in a nucleosome-free state. The structure consists of a stable triangular base module and a flexible Arp module. The conserved subunits Swi1 and Swi3 form the backbone of the complex and closely interact with other components. Snf6, which is specific for yeast Swi/Snf complex, stabilizes the binding of the ATPase-containing subunit Snf2 to the base module. Comparison of the yeast Swi/Snf and RSC complexes reveals conserved structural features that govern the assembly and function of these two subfamilies of chromatin remodelers. Our findings complement those from recent structures of the yeast and human chromatin remodelers and provide further insights into the assembly and function of the SWI/SNF remodelers.

Source: Nature Communications

DOI: 10.1038/s41467-020-17229-x

Authors: Chengcheng Wang, Zhouyan Guo, Xiechao Zhan, Fenghua Yang, Mingxuan Wu, Xiaofeng Zhang

Institutions: Westlake University, Zhejiang University, Center for Life Sciences, Tsinghua University

Language: English

Topic: Chromatin Remodeling and Cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $5,380

Funders: Westlake University, National Natural Science Foundation of China