Enzyme Sampling: Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry (Small 26/2020)
Year: 2020
Type: article
Abstract: In article number 2000584, Milan Mrksich, Horacio D. Espinosa, and co-workers report on a method for non-destructively sampling enzymes multiple times from small populations of cells, using localized electroporation in microfluidic live cell analysis devices. The enzymes are captured in extraction chambers, where they modify immobilized substrates on a self-assembled monolayer, and their activity is quantified using self-assembled monolayers for matrix-assisted laser desorption/ionization (SAMDI) mass spectrometry.
Source: Small
Institution: Northwestern University
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W3039614491