Work

A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field

Year: 2020

Type: review

Abstract: Proteomics is the field of study that includes the analysis of proteins, from either a basic science prospective or a clinical one. Proteins can be investigated for their abundance, variety of proteoforms due to post-translational modifications (PTMs), and their stable or transient protein-protein interactions. This can be especially beneficial in the clinical setting when studying proteins involved in different diseases and conditions. Here, we aim to describe a bottom-up proteomics workflow from sample preparation to data analysis, including all of its benefits and pitfalls. We also describe potential improvements in this type of proteomics workflow for the future.

Source: Proteomes

DOI: 10.3390/proteomes8030014

Authors: Emmalyn J. Dupree, Madhuri Jayathirtha, Hannah Yorkey, Marius Mihasan, Brindusa Alina Petre, Costel C. Darie

Institutions: Clarkson University, Alexandru Ioan Cuza University, Institutul Regional de Oncologie

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,056