Year: 2017
Type: article
Abstract: Background: A recent publication described enrichment of the latent proviral reservoir in resting CD4+ T cells expressing the receptor CD32.Identification of a marker that distinguishes the minority of cells harboring latent HIV-1 provirus from the pool of uninfected CD4+ T cells would represent a crucial scientific advance.Using cells obtained from aviremic HIV-1-positive participants, we hypothesized that CD32 expression would identify a CD4+ T cell population enriched for proviral DNA.Methods: Resting CD4+ T (rCD4) cells were purified via negative magnetic bead isolation from peripheral blood mononuclear cells obtained from aviremic HIV-1-positive participants (n=6).CD32positive rCD4 cells were purified via magnetic bead isolation, and quantitative proviral DNA PCR was carried out on CD32-negative, CD32-positive, and total rCD4 cell populations.Changes in CD32 expression upon T cell activation, de novo or reactivated HIV-1 infection were evaluated in rCD4 cells from both HIV-1-positive and negative donors.In parallel, flow cytometric evaluation of T cell activation markers was carried out to assess for co-expression of CD25, CD69 or HLA-DR with CD32.Viral reactivation was quantified in sorted populations.Results: rCD4 cells from aviremic HIV-1-positive donors expressing CD32 did not contain significantly more proviral DNA than the CD32-negative population or total rCD4 cells (repeated measures one-way ANOVA, P=0.48).CD32 expression did not change with T cell activation, viral reactivation from latency or de novo HIV-1 infection.CD32 expression did correlate with expression of the activation marker HLA-DR (R2=0.99,P<0.001), but not with CD69 or CD25.Viral reactivation did not differ between CD32+, CD32-and total CD4 sorted cell populations (P=0.49).Conclusions: CD32 expression on CD4+ T cells from aviremic HIV-1-positive participants was associated with the activation marker HLA-DR but did not identify a population of cells enriched for replication-competent proviral DNA.
Source: Journal of Virus Eradication
Institutions: The University of Sydney, Westmead Institute for Medical Research, Karolinska University Hospital, Karolinska Institutet, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc. (United States), University of California, San Francisco, Centre Hospitalier de l’Université de Montréal, Université de Montréal
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,950
OpenAlex ID: W3040837669