Synthesis, biocompatibility and gene encapsulation of poly(2-Ethyl 2-Oxazoline)-dioleoyl phosphatidylethanolamine (PEtOx-DOPE) and post-modifications with peptides and fluorescent dye coumarin
Year: 2020
Type: article
Abstract: Sevgi Gulyuzab , Duygu Bayramab, Umut Ugur Ozkoseabc , Zeynep Busra Bolatde , Polen Kocakd , Ongun Mehmet Sakaf , Burcu Devrimf , Melek Parlak Khalilyg , Dilek Telcid , Fikrettin Sahind , Salih Özçubukçuh , Esma Sezerb , Mehmet Atilla Tasdeleni , Onur Alpturkb , Asuman Bozkırf & Ozgur Yilmaza* a Materials Institution, Marmara Research Center, TUBITAK, Kocaeli, Turkeyb Faculty of Science and Letters, Department of Chemistry, Istanbul Technical University, Istanbul, Turkeyc Faculty of Science and Letters, Department of Chemistry, Piri Reis University, Istanbul, Turkeyd Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkeye Faculty of Engineering and Natural Sciences, Department of Molecular Biology and Genetics, Istanbul Sabahattin Zaim University, Istanbul, Turkeyf Faculty of Pharmacy, Department of Pharmaceutical Technology, Ankara University, Ankara, Turkeyg Faculty of Arts and Science, Department of Chemistry, Yozgat Bozok University, Yozgat, Turkeyh Faculty of Arts and Science, Department of Chemistry, Middle East Technical University, Ankara, Turkeyi Faculty of Engineering, Department of Polymer Engineering, Yalova University, Yalova, TurkeySupplemental data for this article can be accessed on the publisher’s website.CONTACT Ozgur Yilmaz [email protected] Turkish Scientific and Technological Council Marmara Research Center (TÜBİTAK MAM), Barış Mah. Dr. Zeki Acar Cad. No. 1, P.K. 21, Gebze 41470, Turkey.AbstractLiposome surface modifications serve great potential applications of liposomes, for instance, increasing stability, bioactive liposome conjugates, and targeted drug, gene, and image agent delivery. In this study, novel targeted lipopolymers, peptide 18/peptide 563-poly(2-ethyl-2-oxazoline)-dioleoylphosphatidyl-ethanolamine (P18/P563-PEtOx-DOPE), have been demonstrated to be successfully synthesized. The structures of P18/P563-PEtOx-DOPE were confirmed by FT-IR spectroscopy, GPC, and 1H-NMR. In this strategy, poly(2-ethyl 2-oxazoline)-modified liposomes were firstly constructed with molecular weights of 3,500 and 5,800 Da. Then, we chose PEtOx5800-DOPE because it has been obtained better particle size (88.74 ± 0.6816) according to the DLS results. Then, peptides- and dye-PEtOx lipid-based nanovesicle (LN) were prepared by peptide-18, peptide-563, and 7-mercapto-4-methyl coumarin. Genetic material (pDNA) was encapsulated into the liposomes and evaluated the encapsulation of plasmid DNA with migration by using agarose gel electrophoresis. In vitro cytotoxicity experiment results on prostate cancer and breast cancer cell lines, parallelly with the healthy prostate (PNT1A) and breast (MCF10A) epithelial cell lines, cells showed insignificant toxic effects. Thus, we can suggest a novel PEtOx phospholipid thanks to this article and its integration with ligands, which great potential for gene transfer system.Graphical Abstract
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3040945370