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Intact HIV genomes are enriched in memory T cells with short half-lives

Year: 2019
Type: article
Abstract: Supplement 3): 1-58 5 visualizes viral proviruses, as confirmed by AluPCR.The tracking of viral DNA is highly specific as corroborated by the absence of nuclear signals in presence of drugs that block nuclear import or reverse transcription.Integrated or extrachromosomal can be directly tracked in infected cells.Results: showed a different diffusion coefficient between proviruses and episomal viral forms.Surprisingly, episomal forms consist of two populations that behave with a different diffusion coefficient.Conclusions: Our study in live cells permits to follow the dynamics of HIV genome and transcription in spatio-temporal mode.Future experiments could elucidate mechanisms and factors involved in the transition from a latent virus to an active virus and vice versa in primary CD4+ T cells.
DOI: 10.1016/s2055-6640(20)30082-0
Authors: V. Morcilla, C. Bacchus-Souffan, T. Schlub, M. Fitch, R. Hoh, S. Deeks, M. Hellerstein, J. Mccune, P. Hunt, S. Palmer
Institutions: The University of Sydney, Westmead Institute, Westmead Institute for Medical Research, University of California, San Francisco, University of California, Berkeley, San Francisco General Hospital, Gates Foundation
Language: English
Subfield: Virology
Open Access status: gold
Estimated APC paid: $2,950
OpenAlex ID: W3041404462
FWCI:
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