Get started
Work

Protocol for Cloning Epithelial Stem Cell Variants from Human Lung

Year: 2020
Type: article
Abstract: The plurality of clonogenic cells derived from human lung includes a spectrum of diverse p63+ stem cells responsible for the regeneration of normal epithelial tissue and disease-associated metaplastic lesions. Here, we report protocols for the cloning, expansion, and characterization of these stem cell variants, which in general assist in analyses of stem cell heterogeneity, genome editing, drug screening, and regenerative medicine. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2011), Zuo et al. (2015), and Rao et al. (2020).
DOI: 10.1016/j.xpro.2020.100063
Authors: Wei Rao, Suchan Niroula, Shan Wang, Matthew Vincent, Frank McKeon, Wa Xian
Language: English
Subfield: Oncology
Field: Medicine
Open Access status: gold
Estimated APC paid: $1,550
Funders: U.S. Department of Defense, Cancer Prevention and Research Institute of Texas, National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases
Awards: PATIENT SPECIFIC STRATEGIES FOR TARGETING THERAPY-RESISTANT CELLS, Clonal Reconstruction and Targeting of the Correa Sequence, G4471003212, Patient-Matched Stem Cells of the Barrett's-Dysplasia-Adenocarcinoma Sequence, Pathogenic Heterogeneity in Mucosal Stem Cells in Pediatric Crohn's Disease, Recruitment of Rising Stars, Pro-Inflammatory Stem Cell Variants in Cystic Fibrosis
OpenAlex ID: W3041666060
FWCI: 0.181
Cites: 10
Cited by: 10
Related to: 10