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Growth inhibition by bacterial Cas2Em proteins expressed in mammalian cells.

Year: 2020

Type: article

Abstract: BACKGROUND: ) is sufficient and enough for PGBC. Thus, the potential role of Cas2Em in microbial-host interaction was explored in this study. METHODS: The impacts of Cas2Em on growth of both CHO-K1 and Hela cells were investigated. The subcellular localization and potential molecular target of Ca2Em were studied. RESULTS: The growth of mammalian cells were inhibited by Cas2Em protein via G1 arresting and apoptosis. In addition, we also demonstrated that Cas2Em was tightly associated with nuclear outer membrane and could be immunoprecipitated with 14-3-3γ through a 30 amino acid domain (homology of CLK2). CONCLUSION: Cas2Em significantly suppressed the growth of mammalian cells indicating Cas2 proteins play an important role in mammalian cells.

Source: PubMed

Authors: Xin Yu, Lei Wang, Lin Zou, Mengjie Li, Tiansheng Li, Linlin Hou, Yameng Guo, Danfeng Shen, Guiqin Sun, Di Qu, Xunjia Cheng, Li Chen

Institutions: Shanghai Medical College of Fudan University, Fudan University, Roche (China), Zhejiang Chinese Medical University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W3043095628

FWCI:

Cites: 29

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Related to: 10