MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods
Year: 2020
Type: article
Abstract: Liquid biopsies have become a convenient tool in cancer diagnostics, real-time disease monitoring, and evaluation of residual disease. Yet, the information still encrypted in the variety of tumor-derived molecules identified in biofluids has proven difficult to decipher due to the technological limitations imposed by their biological nature. Such is the case of extracellular vesicle (EV) encapsulated ncRNAs, which have gained traction in recent years as biomarkers. Due to their resilience towards degrading factors they may act as suitable disease indicators. This review addresses the less described issues in this context. We present an overview of less investigated biofluids that can be used for EV isolation in addition to different isolation approaches to overcome the technical challenges these specimens harbor. Furthermore, we summarize the latest technological advances providing improvement to ncRNA detection and analysis. Thereby, this review summarizes the current state-of-the-art methodologies regarding EV and EV derived miRNA analysis and how they compare to current approaches.
Source: Cancers
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,324
Funders: U.S. Department of Defense, CLL Global Research Foundation, European Commission, Deutsche Forschungsgemeinschaft, Deutsche Krebshilfe, National Institutes of Health, Horizon 2020 Framework Programme, National Cancer Institute, National Institute of General Medical Sciences, National Center for Advancing Translational Sciences, Moonshot Research and Development Program, Common Fund
Awards: Excellence in research and development of non-coding RNA DIAGnostics in ONcology, G1125512807, G1573574303, G1620553470, G2226474896, G24587324, G2663453512, G3195654104, G3705701679, G3733174406, G3764280132, G4298141496, G434337789, G4377847664, G446918063, G5086248425, Protein-coding and non-coding RNA biomarkers for early detection of CLL, G5306837625, G6077865511, UPR/MDACC: Partnership for Excellence in Cancer Research (2 of 2), G6332391933, G6344965762, G6368332692, G6386767959, G6439468129, G6836096280, G6921649073, G6975828589, G7237298611, G755165024, Cancer treatment and monitoring through identification of circulating tumour cells and tumour related nucleic acids in blood, miR-155 targeted therapeutics for precision medicine in lung cancer, G7905850378, G8274656085, G9000059499, G9000065446, G9000157622, G9000183083
OpenAlex ID: W3044032435