Year: 2020
Type: article
Abstract: Stimulation of naive T lymphocytes via the T cell receptor (TCR) induces distinct phosphorylation patterns that can be used to explore various signaling pathways within the cell. This protocol can be used to characterize different perturbations to the signaling pathways and the variations in time of stimulation. Here, we provide a method of barcoding and consolidating a maximum of 24 different sample conditions using two florescent dyes. This single sample for phospho-staining and flow cytometry saves time and reagents. For complete details on the use and execution of this protocol, please refer to Krutzik and Nolan (2006), Krutzik et al. (2012), Vercoulen et al. (2017), Ksionda et al. (2018), and Myers et al. (2019).
Source: STAR Protocols
Institution: University of California, San Francisco
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,550
Awards: G1611259798, G3506794757, G3609659413, G4219210399, Molecular understanding of leukemic bone marrow cytokine-Ras signals and metabolic dependence, G705477112, Loss of Intrinsic Control in Autoimmune T Helper Cells with Signaling Variants, Balanced signaling cues to guide cell transitions in the blood lineage continuum, G8419437111, Defining the Unique Properties of the Distinct Signaling Machinery Used by the TCR
OpenAlex ID: W3044604752