Work

Exosomes from HIV-1 infected cells stimulate production of pro-inflammatory cytokines through TAR RNA.

Year: 2015

Type: article

Abstract: CD4+ T cell line (A3.01) and HIV-1 latently infected cells (ACH2, J1.1, and NCHA1) were aligned to hg18 using bowtie and analyzed with SICER, CEAS, HOMER, WebGestalt, and PCViz.Results: Chromosomes 16, 17, 19 and 22 were significantly enriched for histone modifications in both decreased and increased islands.38 decreased islands from 126 H3K4me3 islands and 302 H3K9ac islands common at specific chromosomes in HIV-1 latent cells and 41 increased islands from 130 H3K4me3 islands and 164 H3K9ac islands were selected for functional annotation.In Gene Ontology analysis, 38 genes were involved in regulation of biological process, regulation of cellular process, biological regulation and purinergic receptor signaling pathway.41 genes were in nucleic acid binding, calcium activated cation channel activity, DNA binding and zinc ion binding.By pathway commons analysis in WebGestalt, 38 genes were strongly involved in p63 transcription factor network and 41 genes were in RNA polymerase Ⅲ transcription termination pathway.TOP2A, ITGB4, TRAF4, SEC14L2, NFIC and NFIX were selected as candidates for HIV latency.Additionally, TRAF4 was found to directly interact with NFIX.Conclusions: The unique enrichment of histone modifications and its cross-talk in specific chromosomes might play a crucial role in the establishment and maintenance of HIV-1 latency.

Source: Journal of Virus Eradication

DOI: 10.1016/s2055-6640(20)31354-6