Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach
Year: 2020
Type: article
Abstract: Significance Traditional genetic modulation approaches are typically restricted to binary perturbations, single-sided titrations (either graded up- or down-regulation), or individual gene expression modulation. Here, we utilize a library approach that is able to simultaneously modulate gene expression in a metabolism-wide manner. This library, when coupled with next-generation sequencing, allows for the identification of gene perturbations that would have been missed by classic approaches. For the examples tested, this library identified targets that improved growth on alternative carbon sources as well as improved production of betaxanthins.
Authors: Emily K. Bowman, Matthew Deaner, Jan-Fang Cheng, Robert Evans, Ernst Oberortner, Yasuo Yoshikuni, Hal S. Alper
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
Awards: G1224080286, G779141928
OpenAlex ID: W3044988264