Work

Phenol-based RNA extraction from polycarbonate filters v1

Year: 2020

Type: preprint

Abstract: This protocol is an acid-phenol-based method for extracting RNA from samples collected onto polycarbonate filters. Bead-beating is included to increase yields from difficult to lyse cells, for example, in some species of cyanobacteria. We have used it successfully in RNA-sequencing projects involving lab cultures of cyanobacteria and from freshwater and marine environmental samples.

DOI: 10.17504/protocols.io.bivuke6w

Authors: Robbie M. Martin, Steven W. Wilhelm

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W3045893196

Cites:

Cited by: 7

Related to: 10