Work

Benzoate Synthesis from Glucose or Glycerol Using Engineered Pseudomonas taiwanensis

Year: 2020

Type: article

Abstract: Benzoic acid is one of the most commonly used food preservatives, but currently exclusively produced in petrochemical processes. In this study, a bio-based production pathway using an engineered strain of Pseudomonas taiwanensis is described. In a phenylalanine-overproducing strain, bacterial and plant genes are heterologously expressed to achieve production of benzoate via a β-oxidation pathway. Strategic disruption of the native Pseudomonas benzoate degradation pathway further allows the production of catechol and cis,cis-muconate. Taken together, this work demonstrates new routes for the microbial production of these industrially relevant chemicals from renewable resources.

Source: Biotechnology Journal

DOI: 10.1002/biot.202000211

Authors: Maike Otto, Benedikt Wynands, Jan Marienhagen, Lars M. Blank, Nick Wierckx

Institutions: Forschungszentrum Jülich, RWTH Aachen University

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Responsible consumption and production

Open Access status: hybrid

Estimated APC paid: $3,800