Omeprazole, an inhibitor of proton pump, suppresses De novo lipogenesis in gastric epithelial cells

Year: 2020

Type: article

Abstract: BACKGROUND: De novo lipogenesis (DNL) has been reported to involve in a serial types of disease. A standard triple therapy, including a PPI, omeprazole, and antibiotics (clarithromycin and amoxicillin), is widely used as the first-line regimen for helicobacter pylori (H. pylori)-infectious treatment. The objective of this study is to explore the function of a standard triple therapy on DNL. METHODS AND RESULTS: We collected the clinical sample from the patients diagnosed with or without H. pylori infection. Oil red staining, real-time PCR, western blotting (WB) and adipored experiment were performed to detect the effect of a standard triple therapy on DNL. The expression of relative key enzymes was assessed in gastric mucosa of clinical sample by IHC. Both 54 cases with H. pylori-negative and 37 cases with H. pylori-positive were enrolled in this study, and IHC assay showed that both fatty acid synthase (FASN) and ATP-citrate lyase (ACLY) expression, the critical enzymes involved in DNL, were increased in gastric mucosa of patients with H. pylori-positive compared with that with H. pylori-negative. Real-time PCR and WB analysis showed that neither clarithromycin nor amoxicillin inhibited FASN and ACLY expression, while treatment of BGC823 cells with omeprazole with 200 μM or 300 μM significantly abolished FASN and ACLY expression, leading to reduce lipid content. CONCLUSION: These findings suggested that omeprazole suppressed DNL in gastric cells, implying that targeting DNL is an alternative strategy in improving the treatment of patients with H. pylori infection.

Source: Biomedicine & Pharmacotherapy

DOI: 10.1016/j.biopha.2020.110472

Authors: Peiyu Chen, Linkai Li, Hongli Wang, Junhong Zhao, Yang Cheng, Jing Xie, Meiwan Cao, Ling Huang, Fangying Yang, Huan Chen, Jiayu Chen, Mingmin Su, Yuxin Xu, Fengfeng Zheng, Lanlan Geng, Wanfu Xu, Sitang Gong

Institutions: Jinan University, Guangzhou Women and Children Medical Center, First Affiliated Hospital of Jinan University, Guangzhou Medical University, Cardiff University, Fujian Medical University, Putian University

Language: English

Topic: Cancer, Lipids, and Metabolism

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,000

Funders: National Natural Science Foundation of China, Sun Yat-sen University, Natural Science Foundation of Guangdong Province, Natural Science Foundation of Hainan Province, Guangzhou Municipal Health and Family Planning Commission, Guangzhou Institute of Pediatrics, Guangzhou Women and Childrens Medical Center, Medical Science and Technology Foundation of Guangdong Province, Guangzhou Municipal Science and Technology Project

OpenAlex ID: W3046134418