Year: 2020
Type: preprint
Abstract: Since the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there have been demands on the testing infrastructure that have strained testing capacity. As a simplification of method, we confirm the efficacy of RNA extraction-free RT-qPCR and saline as an alternative patient sample storage buffer. In addition, amongst potential reagent shortages, it has sometimes been difficult to obtain inactivated viral particles. We have therefore also characterized armored SARS-CoV-2 RNA from Asuragen as an alternative diagnostic standard to ATCC genomic SARS-CoV-2 RNA and heat inactivated virions and provide guidelines for its use in RT-qPCR.
Source: medRxiv
Institution: The University of Texas at Austin
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G3969927967, RAPID: Development of Rapid Point of Care SARS-CoV-2 Detection System, Directed evolution of polymerases that can read and write extremely long sequences, Non-coding RNA and ADP-ribosylation in Antiviral Defense, Elucidating How Tri-phosphatase DUSP11 Controls HCV Infection and Hepatocyte Inflammation
OpenAlex ID: W3047007185