Work

Isolation of lipid-free C-reactive protein by affinity chromatography.

Year: 1989

Type: article

Abstract: Human C-reactive protein purification has been hampered by its association with lipids. Isolation of pure lipid-free C-reactive protein was obtained by a three step procedure. First, partially lipid-free C-reactive protein was obtained by affinity chromatography from ascitic fluids; second, lipid-bound proteins were eliminated by calcium-dependent precipitation; and third, lipid-free pure C-reactive protein was obtained by affinity re-chromatography of the supernatant. A 46-50% yield of lipid-free C-reactive protein was obtained compared with the 14.7% obtained by the old method of extraction with lipid solvents.

Source: PubMed

Author: J M Coll

Institution: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

Language: English

Topic: Biomarkers in Disease Mechanisms

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W30473567

FWCI:

Cites: 33

Cited by:

Related to: 10