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Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations

Year: 2020

Type: article

Abstract: Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent developments from organic synthesis, molecular biotechnology and reaction engineering viewpoints that are devoted to bring these enzymes in industrial applications. This covers natural diversity from different sources, protein engineering strategies for expression, substrate scope, activity and selectivity, stabilisation of enzymes via immobilisation, and the use of peroxygenases in low water media. We believe that peroxygenases have much to offer for selective oxyfunctionalisations and we have much to study to explore the full potential of these versatile biocatalysts in organic synthesis.

Source: Biotechnology Advances

DOI: 10.1016/j.biotechadv.2020.107615

Authors: Markus Hobisch, Dirk Holtmann, Patricia Gomez de Santos, Miguel Alcalde, Frank Hollmann, Selin Kara