Work

Assignment of Backbone Resonances in a Eukaryotic Protein Kinase – ERK2 as a Representative Example

Year: 2011

Type: book-chapter

Abstract: A first step toward the analysis of the structure, dynamics, and interactions of proteins by NMR is obtaining an acceptable level of resonance assignments. This process is nontrivial in most eukaryotic kinases given their size and suboptimal behavior in solution. Using inactive ERK2 as a representative example, we describe the procedures we utilized to achieve a significant degree of completeness of backbone resonance assignment.

Source: Methods in molecular biology

DOI: 10.1007/978-1-61779-480-3_19

Authors: Andrea Piserchio, Kevin N. Dalby, Ranajeet Ghose

Institutions: City College of New York, The University of Texas at Austin, The Graduate Center, CUNY, City University of New York

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Center for Research Resources, National Institute on Minority Health and Health Disparities