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Efficient Multi‐Allelic Genome Editing of Primary Cell Cultures via CRISPR‐Cas9 Ribonucleoprotein Nucleofection

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Year: 2020
Type: article
Source: Current Protocols in Stem Cell Biology
DOI: 10.1002/cpsc.126
Authors: Pia Hoellerbauer, Megan Kufeld, Patrick J. Paddison
Institutions: University of Washington, Fred Hutch Cancer Center, Seagen (United States)
Language: English

Topic: CRISPR and Genetic Engineering
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
SDG: Good health and well-being

Open Access status: closed

Funders: National Science Foundation, American Cancer Society, Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation, National Institutes of Health, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases
Awards: G138319795, G1497121669, G1647654089, G1774909394, G2229826340, MYC-dependent loss of splicing fidelity in Glioblastoma multiforme, G349328344, Core Center of Excellence in Hematology, G9000107151, Cancer Center Support Grant, Stem Cell and Transplantation Biology, Interdisciplinary Training in Cancer Research
OpenAlex ID: W3080947033
FWCI: 0.346
Cites: 52
Cited by: 13
Related to: 10
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