Year: 2021
Type: article
Abstract: Tailocins are phage-derived bacteriocins that demonstrate great potential as agricultural antimicrobials given their high killing efficiency and their precise strain-specific targeting ability. Our group has recently categorized and characterized tailocins produced by and tailocin sensitivities of the phytopathogen Pseudomonas syringae , and here we extend these experiments to test whether prophylactic tailocin application can prevent infection of Nicotiana benthamiana by P. syringae pv. syringae B728a. Specifically, we demonstrate that multiple strains can produce tailocins that prevent infection by strain B728a and engineer a deletion mutant to prove that tailocin targeting is responsible for this protective effect. Lastly, we provide evidence that heritable resistance mutations do not explain the minority of cases where tailocins fail to prevent infection. Our results extend previous reports of prophylactic use of tailocins against phytopathogens, and establish a model system with which to test and optimize tailocin application for prophylactic treatment to prevent phytopathogen infection.
Source: Phytopathology
Authors: David A. Baltrus, Meara Clark, Kevin L. Hockett, Madison Mollico, Caitlin Smith, Savannah Weaver
Institutions: University of Arizona, Pennsylvania State University
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W3183203029