Spatially Targeted Proteomics of the Host–Pathogen Interface during Staphylococcal Abscess Formation
Year: 2020
Type: article
Abstract: is a common cause of invasive and life-threatening infections that are often multidrug resistant. To develop novel treatment approaches, a detailed understanding of the complex host-pathogen interactions during infection is essential. This is particularly true for the molecular processes that govern the formation of tissue abscesses, as these heterogeneous structures are important contributors to staphylococcal pathogenicity. To fully characterize the developmental process leading to mature abscesses, temporal and spatial analytical approaches are required. Spatially targeted proteomic technologies such as micro-liquid extraction surface analysis offer insight into complex biological systems including detection of bacterial proteins and their abundance in the host environment. By analyzing the proteomic constituents of different abscess regions across the course of infection, we defined the immune response and bacterial contribution to abscess development through spatial and temporal proteomic assessment. The information gathered was mapped to biochemical pathways to characterize the metabolic processes and immune strategies employed by the host. These data provide insights into the physiological state of bacteria within abscesses and elucidate pathogenic processes at the host-pathogen interface.
Source: ACS Infectious Diseases
Institution: Vanderbilt University
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Awards: Characterization of the host-pathogen interface during staphylococcal abscess formation, The Staphylococcus aureus response to nutrient zinc restriction during infection, Defining the impact of host factors on the molecular architecture and bacterial physiology of Staphylococcus aureus abscesses, G6293910052, Mechanism and function of heme-iron utilization in staphylococcal pathogenesis, G9000285703, G9000327158, Chemical Biology of Infectious Diseases (CBID) Training Program, Pathobiology of heme inducible transporters in Gram positive pathogens, Molecular mapping of microbial communities at the host-pathogen interface by multi-modal 3-dimensional imaging mass spectrometry, Training Program in Environmental Toxicology, Imaging Mass Spectrometry Research Resource at Vanderbilt University
OpenAlex ID: W3108388539