Work

Advances in Antibody Preparation Techniques for Immunoassays of Total Aflatoxin in Food

Year: 2020

Type: review

Abstract: Aflatoxin (AF) contamination is a major concern in the food and feed industry because of its prevalence and toxicity. Improved aflatoxin detection methods are still needed. Immunoassays are an important method for total aflatoxin (TAF) analysis in food due to its technical advantages such as high specificity, sensitivity, and simplicity, but require high-quality antibodies. Here, we first review the three ways to prepare high-quality antibodies for TAF immunoassay, second, compare the advantages and disadvantages of antigen synthesis methods for B-group and G-group aflatoxins, and third, describe the status of novel genetic engineering antibodies. This review can provide new methods and ideas for the development of TAF immunoassays.

Source: Molecules

DOI: 10.3390/molecules25184113

Authors: Yanan Wang, Jinqing Jiang, Hanna Fotina, Haitang Zhang, Junjie Chen

Institutions: Sumy National Agrarian University, Henan Institute of Science and Technology

Language: English

Topic: Mycotoxins in Agriculture and Food

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $2,112