Glutathione-S-transferase Fusion Protein Nanosensor
Year: 2020
Type: article
Abstract: Fusion protein tags are widely used to capture and track proteins in research and industrial bioreactor processes. Quantifying fusion-tagged proteins normally requires several purification steps coupled with classical protein assays. Here, we developed a broadly applicable nanosensor platform that quantifies glutathione-S-transferase (GST) fusion proteins in real-time. We synthesized a glutathione-DNA-carbon nanotube system to investigate glutathione-GST interactions via semiconducting single-walled carbon nanotube (SWCNT) photoluminescence. We found that SWCNT fluorescence wavelength and intensity modulation occurred specifically in response to GST and GST-fusions. The sensor response was dependent on SWCNT structure, wherein mod ( n – m, 3) = 1 nanotube wavelength and intensity responses correlated with nanotube diameter distinctly from mod ( n – m, 3) = 2 SWCNT responses. We also found broad functionality of this sensor to diverse GST-tagged proteins. This work comprises the first label-free optical sensor for GST and has implications for the assessment of protein expression in situ, including in imaging and industrial bioreactor settings.
Source: Nano Letters
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: Anna Fuller Fund, American Cancer Society, American Heart Association, Ovarian Cancer Research Fund, Honorable Tina Brozman Foundation, Memorial Sloan-Kettering Cancer Center, Society of Memorial Sloan Kettering, Tow Foundation, Pershing Square Sohn Cancer Research Alliance, Expect Miracles Foundation, National Institutes of Health, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke, National Institute of Child Health and Human Development, Division of Chemical, Bioengineering, Environmental, and Transport Systems, National Center for Advancing Translational Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: Tumor-Selective Delivery Approaches for Medulloblastoma, G2295061683, G2377374449, G372934033, Transient Metabolite Detection for Single-Cell Metabolomics and Diagnostics, P-selectin-Mediated Targeting of PI3K Nanomedicines to the Tumor Microenvironment, G6267692869, G6793457528, G6812410181, Targeting Acute Kidney Injury with Mesoscale Nanoparticle Therapeutics, Renal tubule-specific nanotherapeutics for acute kidney injury, G8525765020, Cancer Center Support Grant, Clinical and Translational Science Center
OpenAlex ID: W3087000431