Work

Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson’s Disease Diagnostics

Year: 2020

Type: editorial

Abstract: The egress of α-synuclein in neuronally derived exosomes predates the clinical presentation of Parkinson's disease (PD), offering a means of developing a predictive or prognostic test. Here, we report the reagentless impedimetric assay of two internal exosome markers (α-synuclein and syntenin-1) from neuronal exosomes. Exosomes were efficiently extracted from patient sera using anti-L1CAM conjugated zwitterionic polymer-modified magnetic beads prior to lysis and analyzed by electrochemical impedance spectroscopy. The quantification of α-synuclein level across 40 clinical samples resolved statistically significant differences between PD patients and healthy controls (HC).

Source: Analytical Chemistry

DOI: 10.1021/acs.analchem.0c03092

Authors: Ying Fu, Cheng Jiang, George K. Tofaris, Jason J. Davis

Institutions: University of Oxford, John Radcliffe Hospital

Language: English

Topic: Electrochemical sensors and biosensors

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $5,000